Monitoring fungi cultivation
Motivation
In bioprocesses the morphology of fungi culture is crucial for the enzyme production efficiency. In the growing phase, extensive branching of the fungi takes place. For most species, agglomeration as a result of unfavorable process conditions is detrimental and should be avoided.
Implementation
A Pixact Bioprocess Monitoring (PBM) system is installed to a bioreactor using an 25mm inlet on the reactor side wall. Image data produced by the system is used to analyze the morphology of the fungi culture in real time to observe branching and potential agglomeration of the culture. In addition, information of the gas dispesion is obtained.
Benefits
With real-time image and numerical data from the process, the operator can follow the cultivation in real time and react on unwanted development in morphology.
Process parameters
| Bioprocess type | Trichoderma cultivation |
| Size distribution | 20…300µm |
| Solids concentration | Up to 30 w-% |
| Temperature | 20-30°C |
| Pressure | Atm |
| Other remarks | FDA compatible |